Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Methylation pattern of mouse mitochondrial DNA.

Y Pollack, J Kasir, R Shemer

Nucleic Acids Research

|June 25, 1984

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A randomized study of 6 versus 3 years of adjuvant imatinib in patients with localized GIST at high risk of relapse.

Annals of oncology : official journal of the European Society for Medical Oncology·2024

Same author

S-adenosylmethionine blocks tumorigenesis and with immune checkpoint inhibitor enhances anti-cancer efficacy against BRAF mutant and wildtype melanomas.

Neoplasia (New York, N.Y.)·2023

Same author

Building knowledge, optimising physical and mental health and setting up healthier life trajectories in South African women (<i>Bukhali</i>): a preconception randomised control trial part of the Healthy Life Trajectories Initiative (HeLTI).

BMJ open·2022

Same author

Circulating breast-derived DNA allows universal detection and monitoring of localized breast cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2020

Same author

[Medication reconciliation in a department of internal medicine and infectious and tropical diseases: Feedback after one year practice].

La Revue de medecine interne·2018

Same author

Associations among oxytocin receptor gene (OXTR) DNA methylation in adulthood, exposure to early life adversity, and childhood trajectories of anxiousness.

Scientific reports·2017

Mouse mitochondrial DNA (mtDNA) contains 5-methylcytosine (m5Cyt) primarily at CpG sites. This DNA methylation pattern is nonrandom and resembles vertebrate nuclear DNA, suggesting a potential functional link.

Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

Mitochondrial DNA (mtDNA) plays a crucial role in cellular energy production.
DNA methylation is a key epigenetic mechanism regulating gene expression in nuclear genomes.
The methylation status of mtDNA in mammals, particularly mice, is not fully understood.

Purpose of the Study:

To investigate the pattern and characteristics of DNA methylation in mouse mitochondrial DNA (mtDNA).
To determine the frequency and location of 5-methylcytosine (m5Cyt) within mouse mtDNA.
To compare the methylation patterns of mtDNA with those of vertebrate nuclear DNA.

Main Methods:

Utilized sensitive analytical procedures to detect 5-methylcytosine (m5Cyt) in mouse mtDNA.
Employed restriction enzyme digestion (MspI/HpaII) to assess methylation at specific CpG sites.

Related Experiment Videos

Analyzed the known base composition and nucleotide sequence of mouse mtDNA.

Main Results:

Mouse mtDNA contains 5-methylcytosine (m5Cyt) exclusively at CpG dinucleotides, with a frequency of 3-5%.
Methylation of specific MspI (HpaII) sites varied, indicating a nonrandom methylation pattern in mtDNA.
The CpG dinucleotide sequence is underrepresented in mouse mtDNA, similar to vertebrate nuclear DNA.

Conclusions:

Mouse mtDNA exhibits distinct methylation features, including CpG methylation, site-specific partial methylation, and CpG underrepresentation.
These characteristics of mtDNA methylation mirror those found in vertebrate nuclear DNA.
The observed similarities suggest a potential functional relationship between mitochondrial and nuclear genomes regarding DNA methylation.