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Related Experiment Videos

Persistent in vitro infection with mouse hepatitis virus 3.

L Lamontagne, J M Dupuy

    Advances in Experimental Medicine and Biology
    |January 1, 1984
    PubMed
    Summary

    Mouse hepatitis virus 3 (MHV3) establishes persistent infections in mouse cell cultures, leading to viral carrier states. These persistent infections alter the virus, reducing its ability to cause acute disease in mice.

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    Area of Science:

    • Virology
    • Immunology
    • Cell Biology

    Background:

    • Mouse hepatitis virus 3 (MHV3) exhibits varied pathogenicity across different mouse strains.
    • Understanding MHV3 infection dynamics in vitro is crucial for correlating with in vivo observations.

    Purpose of the Study:

    • To investigate persistent MHV3 infection in mouse embryonic fibroblasts and lymphoid cell lines.
    • To characterize the biological properties of MHV3 variants emerging from persistent infections.

    Main Methods:

    • Infection of primary mouse embryonic fibroblasts and mouse lymphoid cell lines (YAC).
    • Viral titration, cell membrane antigen detection, and assessment of cytopathic effects (cell lysis).
    • Cell cloning, antibody treatment, and characterization of MHV3 variants (thermosensitivity, pathogenicity).

    Main Results:

    • Persistent MHV3 infection was established in vitro, characterized by high virus production and cell lysis.
    • Viral transmission was confirmed as horizontal, not vertical.
    • MHV3 variants from persistent infections lost their capacity to induce lethal acute disease in susceptible mice, suggesting host factor regulation.

    Conclusions:

    • Persistent MHV3 infection in lymphoid cells creates a viral "carrier state" with equilibrium between virus production and cell permissiveness.
    • MHV3 variants derived from persistent infections exhibit altered pathogenicity, with reduced virulence regulated by host factors.

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