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Gastric surface cell function: potential difference and mucosal barrier.

J M McGreevy

    The American Journal of Physiology
    |July 1, 1984
    PubMed
    Summary

    The surface epithelial cells (SEC) of rabbit gastric mucosa are crucial for maintaining its electrical properties and barrier function. Damage to SEC significantly impacts potential difference and resistance, highlighting their vital role.

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    Area of Science:

    • Gastroenterology
    • Physiology
    • Epithelial Biology

    Background:

    • Rabbit fundic mucosa in Ussing chambers shows gastric gland (GG) deterioration over 4 hours, while the surface epithelial cell (SEC) layer remains intact.
    • The sustained potential difference (PD) and resistance (R) suggest SEC are responsible for these electrical properties.

    Purpose of the Study:

    • To investigate the role of SEC in maintaining the electrical and barrier functions of rabbit gastric mucosa.
    • To determine if SEC integrity is essential for the gastric mucosa's potential difference (PD) and resistance (R).

    Main Methods:

    • Selective anoxia was applied to either the SEC (mucosal N2) or GG (serosal N2) of rabbit fundic mucosa in Ussing chambers.
    • Electrical parameters (PD, R, short-circuit current [Isc]) were measured.
    • Unidirectional fluxes of 36Cl and [3H]mannitol were used to assess transcellular and paracellular transport.

    Main Results:

    • Anoxia of SEC rapidly abolished PD and Isc, and significantly reduced R, while anoxia of GG had no effect.
    • Despite zero PD and Isc during SEC anoxia, chloride flux persisted, with transcellular chloride flux (JClcell) becoming negligible.
    • In control tissues, JClcell correlated strongly with Isc, confirming its dependence on active transport.

    Conclusions:

    • The potential difference (PD) and resistance (R) of rabbit gastric mucosa are primarily functions of the surface epithelial cell (SEC) layer.
    • A healthy SEC layer is essential for the barrier characteristics and electrical integrity of the gastric mucosa.
    • SEC are the main contributors to the short-circuit current (Isc) and active transcellular chloride transport.

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