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Related Experiment Videos

Ghost cells as sorption matrix for virus concentration from water.

R Armon, Y Kott, I Neeman

    Applied and Environmental Microbiology
    |June 1, 1984
    PubMed
    Summary

    Pig erythrocyte membranes effectively concentrate bacteriophages like MS-2 and f2, showing high adsorption and elution rates. This method surpasses traditional beef extract flocculation for viral concentration.

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    Area of Science:

    • Virology
    • Biochemistry
    • Materials Science

    Background:

    • Accurate detection of viruses requires efficient concentration methods.
    • Traditional methods like beef extract flocculation have limitations in efficiency and yield.
    • Developing novel adsorbents is crucial for improving viral detection sensitivity.

    Purpose of the Study:

    • To evaluate pig erythrocyte membranes as an adsorbent for concentrating bacteriophages.
    • To compare the efficacy of erythrocyte membranes with beef extract for viral concentration.
    • To assess the adsorption and elution efficiency for bacteriophages and poliovirus.

    Main Methods:

    • Utilizing pig erythrocyte membranes as adsorbent material.
    • Concentrating bacteriophages (phi x-174, MS-2, f2) and poliovirus from saline solutions.
    • Optimizing adsorption at pH 3.5 and elution at pH 7.0.
    • Comparing results with traditional beef extract flocculation.

    Main Results:

    • Erythrocyte membranes achieved 93-100% adsorption and 92-100% elution for bacteriophages.
    • Beef extract yielded lower adsorption (0-98.8%) and elution (61-86.6%).
    • 100% adsorption and 91-129% elution were observed for poliovirus using erythrocyte membranes.

    Conclusions:

    • Pig erythrocyte membranes are a highly effective adsorbent for concentrating various viruses.
    • This method offers superior performance compared to conventional beef extract techniques.
    • Erythrocyte membranes show promise for sensitive and efficient viral detection applications.

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