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Ghost cells as sorption matrix for virus concentration from water
Abstract:
Pig erythrocyte membranes were used as adsorbent material for the concentration of bacteriophage phi x-174, MS-2, and f2 from 5 ml of saline solution. The adsorption was carried out at pH 3.5, and the elution was carried out at pH 7.0. Compared with adsorption on 3% beef extract, bacteriophage adsorption on erythrocyte ghost cells yielded 93 to 100%, and elution was 92 to 100%, whereas beef extract organic flocculation yielded adsorption of 0 to 98.8% and elution of 61 to 86.6%. The same procedure but with poliovirus LSC-1 gave 100% adsorption and 91 to 129% elution.
Insights
Pig erythrocyte membranes effectively concentrate bacteriophages like MS-2 and f2, showing high adsorption and elution rates. This method surpasses traditional beef extract flocculation for viral concentration.
Area of Science:
- Virology
- Biochemistry
- Materials Science
Background:
- Accurate detection of viruses requires efficient concentration methods.
- Traditional methods like beef extract flocculation have limitations in efficiency and yield.
- Developing novel adsorbents is crucial for improving viral detection sensitivity.
Purpose of the Study:
- To evaluate pig erythrocyte membranes as an adsorbent for concentrating bacteriophages.
- To compare the efficacy of erythrocyte membranes with beef extract for viral concentration.
- To assess the adsorption and elution efficiency for bacteriophages and poliovirus.
Main Methods:
- Utilizing pig erythrocyte membranes as adsorbent material.
- Concentrating bacteriophages (phi x-174, MS-2, f2) and poliovirus from saline solutions.
- Optimizing adsorption at pH 3.5 and elution at pH 7.0.
- Comparing results with traditional beef extract flocculation.
Main Results:
- Erythrocyte membranes achieved 93-100% adsorption and 92-100% elution for bacteriophages.
- Beef extract yielded lower adsorption (0-98.8%) and elution (61-86.6%).
- 100% adsorption and 91-129% elution were observed for poliovirus using erythrocyte membranes.
Conclusions:
- Pig erythrocyte membranes are a highly effective adsorbent for concentrating various viruses.
- This method offers superior performance compared to conventional beef extract techniques.
- Erythrocyte membranes show promise for sensitive and efficient viral detection applications.
