Effect of a new synthetic C-nucleoside (EDTO) on cell proliferation

The growth of Ehrlich ascite tumour cells obtained from NMRI mice and the proliferative response of T-lymphocytes from human peripheral blood in the presence of a mitogen: phytohemagglutinin (PHA) were studied in order to see what effect 2-(alpha and beta-D-erythrophuranosyl)-6,6-dimethyl-4,5,6,7-tetrahydroindol+ ++-4-one (a new synthetic C-nucleoside) had on cell proliferation. The uptake of 3H-thymidine was used as an index of cellular growth. The results show that although this C-nucleoside did not significantly alter the growth of the tumour cells, it did significantly inhibit the proliferation of T-lymphocytes.