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Use of the T lymphocyte colony-forming assay to study human lymphocyte interactions
The role of various mononuclear cell subpopulations necessary for T lymphocyte colony formation was investigated. Neither T cell-enriched nor T cell-depleted populations formed colonies as readily as did unfractionated cells. Addition of small numbers of the T cell-depleted pool greatly enhanced the capacity of the T cell-enriched pool to form colonies. Populations depleted of cells bearing the OKT4 phenotype were deficient in their colony-forming capacity. This assay may prove useful in evaluating patients with subtle abnormalities of mononuclear cell function.
The role of various mononuclear cell subpopulations necessary for T lymphocyte colony formation was investigated. Neither T cell-enriched nor T cell-depleted populations formed colonies as readily as did unfractionated cells. Addition of small numbers of the T cell-depleted pool greatly enhanced the capacity of the T cell-enriched pool to form colonies. Populations depleted of cells bearing the OKT4 phenotype were deficient in their colony-forming capacity. This assay may prove useful in evaluating patients with subtle abnormalities of mononuclear cell function.