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Updated: Aug 17, 2026

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
Published on: February 28, 2019
Parameters influencing chicken T-cell response to mitogens
Abstract:
Several factors significantly influenced lymphocyte response to PHA stimulation in standard cell culture systems. Optimal response was obtained with the lymphocyte concentration at about 1.5 X 10(6) cells. Cultures with or without serum did not differ greatly in PHA response at lower lymphocyte concentrations. However, serum was inhibitory at higher lymphocyte concentrations. Addition of erythrocytes (1-10 X 10(6)) was beneficial for lower cell concentrations but deleterious for higher concentrations. The temperature of incubation giving optimal PHA response was 41 degrees C.
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