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Related Experiment Videos

Plasma C3d/C3 quotient as a parameter for in vivo complement activation.

W Nürnberger, S Bhakdi

    Journal of Immunological Methods
    |November 16, 1984
    PubMed
    Summary

    The C3d/C3 quotient is a more sensitive marker for complement activation in systemic lupus erythematosus (SLE) than C3 or C3d alone, especially when C3 levels are not markedly reduced.

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    Area of Science:

    • Immunology
    • Clinical Chemistry

    Background:

    • Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by immune system dysregulation.
    • Complement system activation is implicated in SLE pathogenesis.
    • Assessing complement activation is crucial for understanding SLE activity.

    Purpose of the Study:

    • To evaluate the diagnostic utility of complement component 3 (C3) and its degradation product C3d in SLE patients.
    • To determine if the C3d/C3 ratio offers improved sensitivity for detecting complement activation in SLE.

    Main Methods:

    • Rocket immunoelectrophoresis and Con-A affinoimmunoelectrophoresis were used to measure plasma C3 and C3d levels.
    • Analysis included 30 healthy donors and 58 SLE patients.
    • C3d/C3 quotients were calculated and compared to normal ranges.

    Main Results:

    • Approximately 50% of SLE patients showed decreased C3 levels.
    • Elevated C3d levels were observed in 55% of SLE patients.
    • The C3d/C3 quotient was abnormal in 70% of SLE patients, indicating significant complement activation.

    Conclusions:

    • The C3d/C3 quotient is a more sensitive indicator of in vivo complement activation in SLE than individual C3 or C3d measurements.
    • This ratio is particularly valuable when total C3 levels are not significantly depressed.
    • The findings suggest the C3d/C3 quotient can aid in monitoring SLE activity.

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