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Apical sodium entry in split frog skin: current-voltage relationship.

J DeLong, M M Civan

    The Journal of Membrane Biology
    |January 1, 1984
    PubMed
    Summary
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    This study demonstrates that the Goldman equation accurately describes sodium entry in frog skin epithelium over a wide range of apical membrane potentials. These findings refine our understanding of electrodiffusion in epithelial sodium transport.

    Area of Science:

    • Physiology
    • Epithelial Transport
    • Ion Channels

    Background:

    • Apical sodium (Na+) entry in frog skin epithelium is typically considered electrodiffusive.
    • Previous studies reported limitations of the constant field equation in fitting the apical sodium current (INa)-membrane potential (psi mc) relationship in frog skin.
    • Re-examination of this relationship is necessary to accurately model epithelial sodium transport.

    Purpose of the Study:

    • To re-evaluate the electrodiffusive nature of apical Na+ entry in frog skin epithelium.
    • To determine if the Goldman equation can accurately fit the INa-psi mc relationship over a broad range of membrane potentials.
    • To assess the validity of using high serosal potassium (K+) for transepithelial measurements of INa-psi mc.

    Main Methods:

    Related Experiment Videos

  • Impaling split frog skins across the basolateral membrane to perform direct measurements.
  • Examining current-voltage relationships at early time endpoints (1-25 msec) after voltage pulses.
  • Comparing measurements under baseline conditions with those using high serosal K+ superfusion.

    • Main Results:

    • The Goldman equation provided excellent fits of the INa-psi mc data over large ranges of apical potential (-200 to +145 mV).
    • Rapid transient responses in psi mc were completed within 0.5 to 3.5 msec.
    • High serosal K+ superfusion altered basolateral membrane potential and reduced transepithelial current, deeming it unsuitable for INa-psi mc relationship determination in this model.

    Conclusions:

    • Apical Na+ entry in frog skin epithelium is accurately described by the Goldman equation over extensive membrane potential ranges.
    • Direct electrophysiological measurements are crucial for accurate assessment of INa-psi mc relationships in tissues like frog skin.
    • Estimated intracellular Na+ concentration and apical Na+ permeability align with previous findings.