Morphology of endoneurial blood vessels of frog sciatic nerve during vascular perfusion

In order to determine if increased injection pressures can alter the permeability and ultrastructure of blood vessels of the frog blood-nerve barrier, these vessels were examined following perfusion of the iliac artery at rates of 0.21 or 0.82 ml/min. At either perfusion rate, endoneurial blood vessel profiles were clearly evident and the surface area of these vessels amounted to 60% of the surface area of the perineurium. In all vessels a large number of vesicles were present within the endothelial cells. Many were attached by necks to one or the other plasma membrane, but no transcellular channels were evident. At the higher flow rate no changes in vesicles or junctions were seen, but blebs and blisters were evident at the luminal membranes of the endoneurial endothelium. When microperoxidase was perfused at 0.82 ml/min, reaction product frequently flooded the endothelial cells, was found as clumps on the cell surface, and was distributed within the endoneurial space. These changes represent the only ultrastructural evidence of endothelial cell damage and altered permeability in response to increased rate of perfusion.