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Related Experiment Videos

Complement in chronic secretory otitis media. C3 breakdown and C3 splitting activity.

S Meri, T Lehtinen, T Palva

    Archives of Otolaryngology (Chicago, Ill. : 1960)
    |December 1, 1984
    PubMed
    Summary

    This study found complement system activation in middle-ear fluid from children with secretory otitis media (SOM). The fluid contained factors that split complement component C3, indicating local immune responses in SOM.

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    Area of Science:

    • Immunology
    • Otolaryngology
    • Biochemistry

    Background:

    • Secretory otitis media (SOM) is a common condition in children.
    • The role of the complement system in SOM pathogenesis is not fully understood.

    Purpose of the Study:

    • To investigate the occurrence of complement component C3 breakdown and C3 splitting activity in middle-ear effusion (MEE) from children with SOM.
    • To identify factors responsible for complement activation within the MEE.

    Main Methods:

    • Analysis of serum and MEE samples from 30 children with SOM.
    • Measurement of C3 breakdown products, factor B, C4, and C3 levels.
    • Assay for C3 splitting activity in MEE using normal human serum.
    • Testing the effect of a classic complement pathway inhibitor.

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    Main Results:

    • MEE showed elevated C3 breakdown products and decreased levels of C3, C4, and factor B.
    • Total hemolytic complement activity was absent in MEE.
    • MEE contained factors that split C3 in vitro, independent of the classic complement pathway.

    Conclusions:

    • Strong local complement activation occurs in the middle ear of patients with SOM.
    • Factors responsible for C3 splitting are present within the MEE, suggesting an intrinsic pathway of activation.