Mouse erythrocyte membrane sialoglycoproteins and their involvement in the interaction with mouse rosette forming...

Sialoglycoproteins were isolated from Balb/c mouse erythrocyte membranes. The crude preparation showed four PAS-positive zones in SDS-polyacrylamide gel electrophoresis. The lowest molecular weight component was separated on the Ultrogel AcA44 column in the presence of SDS. The carbohydrate composition of the fractions obtained indicated that the sialoglycoproteins contained O-glycosidic oligosaccharide chains and and that the lowest molecular weight glycoprotein also contained N-glycosidic chain(s). The sialoglycoproteins strongly and specifically inhibited the rosette formation between mouse erythrocytes and human B lymphocytes. It suggested that sialoglycoproteins were the mouse red cell components involved in the interaction with human lymphocytes.