Changes in lysosomal enzyme activities of eye tissues in endotoxin-induced inflammation

Lysosomal enzyme activities in rabbit ocular tissues were examined at various times after the intravitreal injection of endotoxin. Lysosomal enzyme activities in the aqueous, vitreous, and pigment epithelium-choroid were elevated 3 h after injection. It is deduced from the fluorescein-labeled dextran and SDS-polyacrylamide gel electrophoresis analysis of the aqueous, vitreous, and pigment epithelium-choroid from endotoxin injected rabbits that the elevation of lysosomal enzyme activities are probably due to a breakdown in blood-ocular barriers.