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Updated: Aug 11, 2026

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Published on: April 4, 2011
[Fractions from mouse brain which non-specifically inhibit the hemagglutination of various togaviruses]
1Instituto de Virología, Dr. José M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Abstract:
Brain fractions isolated from Swiss albino mice were studied from the physical, chemical and biological viewpoints. The isoionic point gave a value of 4.16 +/- 0.17 indicating the presence of acid groups. UV absorption experiments showed an opening of the structure when the fractions were heated. The presence of neuraminic acid group was demonstrated using o-phenanthroline and, when heated, the structure exposed the hidden neuraminic acid. The biological property of inhibiting viral hemagglutination increased when the fractions were heated. EEE virus was used as hemagglutinating antigen. The treatment with alpha-chymotrypsin destroyed the inhibiting viral hemagglutination property in 120 seconds. Polyacrylamide gel electrophoresis showed that the fractions are proteic in nature. Glyco- and lipoproteins were only present in the fraction with the inhibitory property. Only neutral lipids were demonstrated.
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