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Related Experiment Videos

Trypsin-activatable inactive renin in rat plasma.

N Glorioso, P Madeddu, P Dessi'-Fulgheri

    Clinical Science (London, England : 1979)
    |February 1, 1983
    PubMed
    Summary
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    Inactive renin in rat plasma requires specific trypsin concentrations and incubation times for activation. Optimal conditions involve 6 mg/ml trypsin for 1 minute at 4°C and pH 6.2, with 40% of total renin being inactive.

    Area of Science:

    • Biochemistry
    • Physiology
    • Renal Physiology

    Background:

    • Inactive renin constitutes a significant portion of total renin in rat plasma.
    • Understanding inactive renin activation is crucial for accurate renin activity measurement.

    Purpose of the Study:

    • To investigate the optimal conditions for activating inactive renin in rat plasma using trypsin.
    • To characterize the interference of trypsin with renin activity assays.

    Main Methods:

    • Incubation of rat plasma with varying trypsin concentrations and durations at 4°C and pH 6.2.
    • Measurement of angiotensin I (ANG I) generation and cleavage of renin substrate fragments.

    Main Results:

    • Maximal inactive renin activation occurred with 6 mg/ml trypsin for 1 minute at 4°C, pH 6.2.

    Related Experiment Videos

  • Trypsin concentrations below 2 mg/ml did not activate inactive renin due to endogenous anti-trypsin activity.
  • Incubation exceeding 2 minutes led to direct cleavage of ANG I-containing fragments, interfering with assays.
  • Angiotensin I formation showed a pH optimum of 5.3 but was unaffected at pH 6.2.

    • Conclusions:

    • Approximately 40% of total renin in normotensive rat plasma is in an inactive form.
    • Precise control of trypsin concentration and incubation time is essential for accurate inactive renin activation and measurement.
    • Extended trypsin incubation can artifactually inflate renin activity measurements.