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Related Concept Videos

Bile01:19

Bile

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Bile is a crucial bodily fluid, characterized by its yellow-green color and alkaline nature. Produced in the liver, it is transported through the common hepatic duct into either the cystic duct, leading to the gallbladder, or directly into the common bile duct. The flow of bile is regulated by the sphincter of Oddi located at the entrance of the duodenum. When this sphincter is closed, bile is redirected to the gallbladder for storage and concentration.
Bile is released when dietary fats enter...
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Amino acids03:42

Amino acids

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Amino acids are the monomers that comprise proteins. Each amino acid has the same fundamental structure, which consists of a central carbon atom, or the alpha (α) carbon, bonded to an amino group (NH2), a carboxyl group (COOH), and to a hydrogen atom. Every amino acid also has another atom or group of atoms bonded to the central atom known as the R group. There are 20 common amino acids present in proteins, each with a different R group. Variation in the amino acid sequence is responsible for...
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Polyprotic Acids03:38

Polyprotic Acids

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Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:
32.0K
Mixtures of Acids03:27

Mixtures of Acids

21.8K
The pH of a solution containing an acid can be determined using its acid dissociation constant and its initial concentration. If a solution contains two different acids, then its pH can be determined using one of several methods depending upon the relative strength of the acids and their dissociation constants.
A Mixture of a Strong Acid and a Weak Acid
In a mixture of a strong acid and a weak acid, the strong acid dissociates completely and becomes a source of almost all the hydronium ions...
21.8K
Nucleic Acids02:43

Nucleic Acids

50.3K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids02:43

Nucleic acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Ileectomy-induced Bile Overaccumulation in Mouse Intestine
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Ileectomy-induced Bile Overaccumulation in Mouse Intestine

Published on: August 21, 2017

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Serum bile acid analysis.

K D Setchell, A Matsui

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |January 7, 1983
    PubMed
    Summary
    This summary is machine-generated.

    This study details advanced methods for analyzing bile acids in serum using liquid-solid extraction and gas chromatography-mass spectrometry. These techniques offer enhanced sensitivity for metabolic profiling in various patient groups.

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    Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
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    Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Clinical Chemistry

    Background:

    • Bile acids are critical biomarkers for liver function and metabolic health.
    • Accurate quantification of serum bile acids is essential for diagnosing and monitoring various diseases.
    • Conventional methods for bile acid analysis often lack the required sensitivity and specificity.

    Purpose of the Study:

    • To outline routine and research techniques for comprehensive serum bile acid analysis.
    • To describe methods for metabolic profiling of bile acids with improved sensitivity.
    • To demonstrate the applicability of these techniques in clinical settings.

    Main Methods:

    • Liquid-solid extraction for sample preparation.
    • Liquid-gel chromatography for isolation and purification.
    • Gas chromatography with high-resolution glass capillary columns and mass spectrometry for sensitive detection.

    Main Results:

    • Developed and validated sensitive methods for serum bile acid quantification.
    • Established rapid and flexible protocols for bile acid metabolic profiling.
    • Demonstrated increased sensitivity compared to conventional measurement techniques.

    Conclusions:

    • The described techniques provide a sensitive and robust approach for serum bile acid analysis.
    • Metabolic profiling of bile acids aids in understanding disease states like liver disease and ileal resection.
    • These advanced analytical methods enhance diagnostic capabilities for hepatobiliary and gastrointestinal disorders.