Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Direct immunofluorescence in pyoderma gangrenosum.

F C Powell, A L Schroeter, H O Perry

    The British Journal of Dermatology
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of Demodex mite viability using motility and scattered light intensity.

    Experimental & applied acarology·2019
    Same author

    Enhancing survival of Demodex mites in vitro.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2018
    Same author

    Demodex mites modulate sebocyte immune reaction: possible role in the pathogenesis of rosacea.

    The British journal of dermatology·2018
    Same author

    Study of Demodex mites: Challenges and Solutions.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2015
    Same author

    Understanding rosacea.

    The British journal of dermatology·2015
    Same author

    Epidermal hydration levels in patients with rosacea improve after minocycline therapy.

    The British journal of dermatology·2013

    Direct immunofluorescence in pyoderma gangrenosum (PG) revealed immune reactant deposition in 61% of cases. Findings suggest a vasculitic pathogenesis for this inflammatory skin condition.

    Area of Science:

    • Dermatology
    • Immunopathology
    • Pathology

    Background:

    • Pyoderma gangrenosum (PG) is a rare, ulcerative inflammatory skin disease.
    • The exact pathogenesis of PG remains incompletely understood.
    • Immune system dysregulation is suspected in PG development.

    Purpose of the Study:

    • To investigate the potential role of immune complex deposition in the pathogenesis of pyoderma gangrenosum.
    • To evaluate the utility of direct immunofluorescence in diagnosing PG.
    • To identify specific immunopathological patterns associated with PG.

    Main Methods:

    • Direct immunofluorescence (DIF) was performed on biopsy specimens from the active, erythematous border of lesions.
    • Fifty-one patients with clinically diagnosed pyoderma gangrenosum were included in the study.

    Related Experiment Videos

  • Immunoreactants (e.g., immunoglobulins, complement) were examined for deposition patterns.

    • Main Results:

    • Positive DIF findings were observed in 31 out of 51 cases (61%) of pyoderma gangrenosum.
    • The most common pattern of deposition was perivascular, seen in 27 cases (53% of total cases).
    • Other less frequent patterns included granular or linear deposition along the basement membrane.

    Conclusions:

    • The presence of immune reactant deposition, particularly perivascularly, supports a vasculitic mechanism in pyoderma gangrenosum.
    • Direct immunofluorescence is a valuable tool for identifying underlying immunopathological processes in PG.
    • These findings contribute to understanding the inflammatory pathways involved in pyoderma gangrenosum.