A method for experimental induction of bronchogenic carcinoma in subcutaneously implanted bronchial autograft in dogs

A method for induction of carcinoma in subcutaneously implanted bronchial autografts in dogs is described. Four to 6 weeks after successful bronchial autografting, 30 to 90 mg. amounts of 3-MC or B(a)P suspended in sterile saline solution containing 1 percent CMC was injected into the lumen of the graft. The mucosal changes were followed histologically by excision of biopsy specimens and cytologically by needle aspiration biopsies. Three dogs developed squamous cell carcinomas invading deep into the submucosal area as early as 13 to 30 weeks after the first carcinogen treatment. The remaining eight experimental dogs showed various grades of squamous cell metaplasia with atypia within 30 weeks. Subsequently examined specimens revealed atypical metaplastic and proliferative changes further advanced than those seen in the initial biopsy specimens. The advantages of this method for a better understanding of the development of bronchogenic carcinomas are discussed.