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Related Experiment Videos

Filament ring formation in the dimorphic yeast Candida albicans.

D R Soll, L H Mitchell

    The Journal of Cell Biology
    |February 1, 1983
    PubMed
    Summary

    The filament ring in Candida albicans is crucial for cell division, forming at specific times and locations during budding and mycelial growth. This structure is closely associated with septum formation, guiding cell shape changes.

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    Area of Science:

    • * Cell biology
    • * Mycology
    • * Microbial pathogenesis

    Background:

    • * Candida albicans exhibits dimorphism, switching between yeast (budding) and hyphal (mycelial) forms.
    • * The mechanisms regulating these morphological transitions and cell division are not fully understood.
    • * Septum formation is a critical event in cell division for fungi.

    Purpose of the Study:

    • * To investigate the role and characteristics of the filament ring during Candida albicans morphogenesis.
    • * To determine the temporal and spatial dynamics of filament ring formation in budding versus mycelial growth.
    • * To explore the relationship between the filament ring and chitinous septum formation.

    Main Methods:

    • * Culturing of Candida albicans stationary phase cells under specific pH conditions (pH 4.5 for budding, pH 6.5 for mycelia) at 37°C.
    • * Microscopic observation and analysis of filament ring formation during different growth phases.
    • * Sectioning and examination of filament ring structures in budding and mycelial cells.

    Main Results:

    • * A filament ring forms under the plasma membrane during both budding and mycelial growth in Candida albicans.
    • * The filament ring appears earlier in budding cells (at mother cell-bud junction) than in mycelial cells (along the elongating hyphae).
    • * Filament rings consist of approximately 11-12 equidistant filaments and disappear as the primary septum forms, suggesting a role in its placement.

    Conclusions:

    • * The filament ring is a key structure involved in regulating cell division and morphogenesis in Candida albicans.
    • * Temporal and spatial differences in filament ring formation correlate with the commitment to budding or mycelial growth.
    • * The filament ring likely plays a role in the positioning and formation of the chitin-containing septum.

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