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[The Homogeneous immunocofactor method of determining insulin]
Prikladnaia Biokhimiia I Mikrobiologiia
|January 1, 1983
Summary
A new quantitative method accurately measures insulin using antibody competitive binding and a novel NAD-insulin conjugate. This assay offers sensitive detection of insulin levels in solution.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunology
Context:
- Accurate insulin quantification is crucial for diagnosing and managing diabetes.
- Existing methods may have limitations in sensitivity or require complex procedures.
Purpose:
- To develop a novel, sensitive, and quantitative immunocofactor assay for measuring insulin in solution.
- To utilize antibody competitive binding with a NAD-insulin conjugate for enhanced detection.
Summary:
- A quantitative immunocofactor method was developed using antibody competitive binding of free and NAD-labeled insulin.
- A NAD-insulin conjugate was synthesized via covalent binding with a modified cofactor and insulin using carbodiimide.
- An enzymatic system for cofactor regeneration was employed to determine unbound NAD-insulin conjugate in the presence of antibodies and free insulin.
Impact:
- The developed assay demonstrates a sensitivity of approximately 5.10(-7) M for insulin detection.
- This method provides a potentially valuable tool for insulin level determination in research and clinical settings.