Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Particulate contamination in disposable corneal trephines.

G J Pardos, D W Meltzer

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |April 1, 1983
    PubMed
    Summary

    Disposable trephines used in corneal transplantation can transfer a black contaminant to donor corneal buttons. Analysis revealed this contaminant is a mix of stainless steel and silicon carbide from the trephine manufacturing process.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Compliance with topical timolol treatment.

    American journal of ophthalmology·1987
    Same author

    Compliance with topical pilocarpine treatment.

    American journal of ophthalmology·1986
    Same author

    Can ophthalmologists correctly identify patients defaulting from pilocarpine therapy?

    American journal of ophthalmology·1986
    Same author

    A miniature compliance monitor for eyedrop medication.

    Archives of ophthalmology (Chicago, Ill. : 1960)·1984
    Same author

    Use of the piezo-electric gun to remove surface charge from intraocular lenses.

    Journal - American Intra-Ocular Implant Society·1984
    Same author

    Microbial contamination of donor eyes. A retrospective study.

    Archives of ophthalmology (Chicago, Ill. : 1960)·1982

    Area of Science:

    • Ophthalmology
    • Materials Science
    • Biomedical Engineering

    Background:

    • Corneal transplantation is a surgical procedure to restore vision by replacing a damaged cornea.
    • Disposable trephines are commonly used surgical instruments for preparing corneal tissue during transplantation.
    • Ensuring the purity and integrity of donor tissue is critical for successful surgical outcomes.

    Observation:

    • A black contaminant was observed on the edges of donor corneal buttons in two separate corneal transplantation cases.
    • The contaminant was traced back to the disposable trephines used during tissue preparation.
    • Trephines from multiple manufacturers exhibited the same contamination issue.

    Findings:

    • Scanning electron microscopy (SEM) and energy-dispersive x-ray analysis (EDX) were employed to identify the contaminant.
    • The contaminant was determined to be a composite material.
    • The material consisted of stainless steel particles originating from the trephine itself and silicon carbide abrasive used in the sharpening process.

    Implications:

    • This finding highlights a potential risk of iatrogenic contamination during corneal transplantation.
    • The presence of metallic and abrasive particles could negatively impact graft survival and visual outcomes.
    • Further investigation into trephine manufacturing and quality control is warranted to prevent donor tissue contamination.

    Related Experiment Videos