Conditionally lethal tubulin mutations of Aspergillus nidulans

Conditionally lethal tubulin mutants are becoming an important tool for studying microtubule structure and function. In the filamentous fungus Aspergillus nidulans it has been possible to isolate heat-sensitive beta-tubulin mutations by isolating mutants resistant to the anti-microtubule agent benomyl and screening these mutants for heat sensitivity. It has also been possible to isolate alpha-tubulin mutations, new beta-tubulin mutations and mutations in other genes whose products are likely to interact with beta tubulin as revertants of heat-sensitive beta-tubulin mutations.