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Revised methods for the Salmonella mutagenicity test.

D M Maron, B N Ames

    Mutation Research
    |May 1, 1983
    PubMed
    Summary
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    This study revises the Salmonella mutagenicity test methods for detecting carcinogens and mutagens. New tester strains and procedural changes improve the accuracy and scope of this essential toxicology assay.

    Area of Science:

    • Toxicology
    • Microbiology
    • Genetics

    Background:

    • The Salmonella mutagenicity assay (Ames test) is a standard method for detecting chemical mutagens.
    • Previous methods require revision to incorporate advancements in strain development and procedural optimization.

    Purpose of the Study:

    • To present a revised protocol for the Salmonella mutagenicity test.
    • To introduce new tester strains and update existing ones for enhanced mutagen detection.
    • To refine procedural aspects including strain maintenance and mutagen selection.

    Main Methods:

    • Introduction of two new tester strains: TA97 (frameshift) and TA102 (ochre mutation on multicopy plasmid).
    • Replacement of strain TA1537 with TA97 in the standard set.
    • Modification of diagnostic mutagens by removing MNNG and 9-aminoacridine.

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  • Minor procedural adjustments in tester strain growth, storage, and preservation.

    • Main Results:

    • The revised Salmonella mutagenicity test incorporates improved tester strains for broader mutagen detection.
    • Specific strains (TA1535, TA1538) are removed from the recommended set, while TA98 and TA100 are retained.
    • Updated procedures enhance the reliability and efficiency of the mutagenicity assay.

    Conclusions:

    • The revised Salmonella mutagenicity test offers an updated and refined approach to mutagenicity testing.
    • The inclusion of new strains and procedural modifications enhances the assay's sensitivity and applicability.
    • These updates ensure the continued relevance of the Salmonella mutagenicity test in toxicological research.