Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in BCG strains.

T W Osborn

    Tubercle
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Bacille Calmette-Guérin (BCG) vaccine strains have diverged due to different maintenance and production methods. Monitoring these changes may reveal insights into the original attenuation process and improve vaccine consistency.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of tampons and menses on the composition and diversity of vaginal microbial communities over time.

    BJOG : an international journal of obstetrics and gynaecology·2013
    Same author

    Use of gene probes based on the insertion sequence IS986 to differentiate between BCG vaccine strains.

    The Journal of applied bacteriology·1992
    Same author

    Effect of the method of preparation of bacille Calmette-Guérin (BCG) vaccine on the properties of four daughter strains.

    The Journal of applied bacteriology·1987
    Same author

    Some effects of nutritional components on the morphology of BCG colonies.

    Developments in biological standardization·1986
    Same author

    What is BCG?

    Tubercle·1983
    Same author

    BCG vaccine: an investigation of colony morphology from four different strains after their introduction as seed for vaccine preparation in four production laboratories.

    Journal of biological standardization·1983
    Same journal

    Trimethylene-glycol-phenol mixture for rapid staining of the tubercle bacillus.

    Tubercle·2010
    Same journal

    Mass x-ray of pregnant women.

    Tubercle·2010
    Same journal

    Extrapleural pneumothorax.

    Tubercle·2010
    Same journal

    Tuberculosis of skeleton and lung combined; prognosis and treatment.

    Tubercle·2010
    Same journal

    Artificial pneumothorax in patients with high blood sedimentation rate.

    Tubercle·2010
    Same journal

    Tuberculosis in Sweden and the fight against it in recent years.

    Tubercle·2010
    See all related articles

    Area of Science:

    • Microbiology
    • Vaccinology
    • Bacteriology

    Background:

    • Bacille Calmette-Guérin (BCG) vaccine, derived from a virulent bovine strain of Mycobacterium bovis, has undergone extensive subculturing.
    • Numerous production centers maintain BCG strains using diverse media and techniques, leading to variations among daughter strains.
    • The mechanisms driving these genetic and phenotypic changes in BCG strains have remained unclear.

    Purpose of the Study:

    • To investigate the relationship between BCG strain variations and the methods used for strain maintenance and vaccine production.
    • To explore potential mechanisms for BCG attenuation and propose strategies for minimizing strain changes.

    Main Methods:

    • Review of existing literature and experimental studies monitoring changes in BCG strains during serial subculture.

    Related Experiment Videos

  • Analysis of different media and techniques employed in BCG vaccine production laboratories.
  • Comparison of observed strain changes with historical data on BCG attenuation.

    • Main Results:

    • Distinct differences have emerged between BCG daughter strains due to varied maintenance protocols.
    • Experimental studies allow for monitoring of changes within BCG strains during subculture.
    • Selection of minority populations within BCG strains may mirror the original attenuation process.

    Conclusions:

    • Different laboratory practices contribute to the divergence of BCG vaccine strains.
    • Understanding these variations is crucial for ensuring vaccine efficacy and consistency.
    • Implementing standardized maintenance and production methods can minimize undesirable changes in BCG strains.