Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein
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Updated: Oct 24, 2026
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Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
Published on: September 17, 2008
The Biochemical Journal
|January 1, 1983
Abstract:
A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.
