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Related Experiment Videos

Intra- and transepithelial analytical techniques.

A D Macknight, J P Leader

    Critical Reviews in Clinical Laboratory Sciences
    |January 1, 1983
    PubMed
    Summary
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    Understanding epithelial transport involves determining driving forces, pathways, and control factors. This review evaluates techniques like microelectrode analysis and microscopy for studying solute and water movement across epithelia.

    Area of Science:

    • Physiology
    • Cell Biology
    • Biophysics

    Background:

    • Epithelial cells are crucial for transporting solutes and water.
    • Studying this transport requires understanding driving forces, pathways, and regulatory factors.
    • Existing methods face challenges in fully characterizing complex transport mechanisms.

    Purpose of the Study:

    • To review and assess the applicability and validity of various techniques for studying epithelial transport.
    • To provide insights into determining driving forces, pathways, and controlling factors of epithelial transport.
    • To guide researchers in selecting appropriate methods for investigating solute and water movement.

    Main Methods:

    • Microelectrode techniques for electrical potential measurements.

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  • Chemical analyses and microprobe analysis for solute composition.
  • Microscopy and metabolic coupling assays for pathway and energy source assessment.

    • Main Results:

    • Transepithelial driving forces are determinable under specific experimental conditions.
    • Paracellular pathway permeability and selectivity can be defined using defined manipulations.
    • Cellular pathway analysis requires assessing apical and basolateral membrane properties and energy coupling.

    Conclusions:

    • A comprehensive understanding of epithelial transport necessitates evaluating multiple techniques.
    • The choice of method depends on whether transport is paracellular or transcellular.
    • Assessing energy sources is vital for understanding active transport mechanisms across epithelia.