Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acid alpha-glucosidase from human heart.

J P Chambers, J C Williams

    Enzyme
    |January 1, 1983
    PubMed
    Summary

    Human heart and liver alpha-glucosidase enzymes were purified and compared. No significant differences in molecular weight or kinetic properties were found between these two alpha-glucosidase enzymes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Determination of |Vub| from measurements of the electron and neutrino momenta in inclusive semileptonic B decays.

    Physical review letters·2005
    Same author

    Measurements of branching fractions and time-dependent CP-violating asymmetries in B --> eta'K decays.

    Physical review letters·2005
    Same author

    Search for decays of B0-->e+ e-, B0-->mu+ mu-, B0-->e +/- mu-/+.

    Physical review letters·2005
    Same author

    Branching fraction and CP asymmetries of B0-->K0(S)K0(S)K0(S).

    Physical review letters·2005
    Same author

    Search for lepton flavor violation in the decay tau+/- --> mu+ gamma.

    Physical review letters·2005
    Same author

    Search for the rare leptonic decay B--->tau-nutau.

    Physical review letters·2005

    Area of Science:

    • Biochemistry
    • Enzymology

    Background:

    • Alpha-glucosidase enzymes play crucial roles in carbohydrate metabolism.
    • Investigating tissue-specific variations in enzyme properties is essential for understanding metabolic pathways.

    Purpose of the Study:

    • To purify and characterize human cardiac alpha-glucosidase.
    • To compare the properties of cardiac alpha-glucosidase with purified human liver alpha-glucosidase.

    Main Methods:

    • Purification of alpha-glucosidase from human heart (2,600-fold).
    • Comparative analysis of molecular weight, cation effects, thermal stability, kinetic properties (affinity and hydrolysis velocity constants), and inhibition by turanose for both enzymes.

    Main Results:

    • Purified human cardiac alpha-glucosidase exhibits maximal activity at acid pH.
    • No significant differences were observed in molecular weight between cardiac and hepatic alpha-glucosidase.
    • Kinetic properties, including substrate affinity and hydrolysis rates for maltose, glycogen, and 4-methylumbelliferyl-alpha-D-glucose, were comparable.

    Conclusions:

    • Cardiac and hepatic alpha-glucosidase enzymes are biochemically similar.
    • These findings suggest a conserved structure and function of alpha-glucosidase across different human tissues.

    Related Experiment Videos