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Cellular factors which modulate hormone responses: glucocorticoid action in perspective
International Review of Cytology. Supplement
|January 1, 1983
Summary
Glucocorticoid effects primarily alter gene transcription via receptor binding. Understanding the receptor's physical nature and location is key to deciphering gene activation processes.
Area of Science:
- Molecular Biology
- Endocrinology
- Genetics
Background:
- Glucocorticoids exert effects mainly by altering gene transcription.
- Steroid binding to receptors and subsequent nuclear translocation are critical steps.
Purpose of the Study:
- To investigate the mechanisms of glucocorticoid-mediated gene transcription.
- To understand the physical properties and cellular localization of the glucocorticoid receptor.
Main Methods:
- The study focuses on the pivotal events of steroid-receptor complex formation and nuclear binding.
- It highlights the need for receptor purification and characterization of nuclear binding sites.
Main Results:
- Glucocorticoid effects are mediated by changes in gene transcription.
- Steroid binding and nuclear translocation are key, but influenced by membrane traversal and receptor activation.
Conclusions:
- The physical nature, subcellular location, and gene activation mechanism of the glucocorticoid receptor remain largely unknown.
- Purifying the receptor and characterizing nuclear binding sites are essential for future research.