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Nonsense mutations in the can1 locus of Saccharomyces cerevisiae
Journal of Bacteriology
|June 1, 1983
Abstract:
Yeast mutants resistant to L-canavanine were selected. All were recessive and fell into the can1 complementation group. Nonsense mutations were identified among them by using a set of different suppressors. Frequencies of UAA, UAG, and presumed UGA mutations were 14.8, 0.8, and 0.4%, respectively. A high incidence of nonsense mutations having discriminatory suppression patterns was characteristic of the locus.
Insights
Researchers identified yeast mutants resistant to L-canavanine, finding that most were nonsense mutations. This study characterizes the can1 locus in yeast, revealing specific mutation frequencies.
Area of Science:
- * Molecular and Cellular Biology
- * Yeast Genetics
Background:
- * L-canavanine is an arginine analog toxic to yeast.
- * Selection for resistance can identify mutations in specific genes.
Purpose of the Study:
- * To characterize yeast mutants resistant to L-canavanine.
- * To identify and classify the types of mutations conferring resistance.
Main Methods:
- * Selection of L-canavanine resistant yeast mutants.
- * Complementation analysis to group mutants.
- * Identification of nonsense mutations using suppressor strains.
Main Results:
- * All selected mutants were recessive and belonged to the can1 complementation group.
- * Nonsense mutations (UAA, UAG, UGA) were identified.
- * Frequencies: UAA (14.8%), UAG (0.8%), UGA (0.4%).
Conclusions:
- * The can1 locus is characterized by a high incidence of nonsense mutations.
- * These mutations exhibit discriminatory suppression patterns, aiding in their identification.