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A simple method for determining polymeric IgA-containing immune complexes.

J Sancho, J Egido, E González

    Journal of Immunological Methods
    |June 10, 1983
    PubMed
    Summary
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    A new assay simplifies the detection of polymeric IgA-immune complexes in biological fluids. This method uses specific secretory component binding and IgM immunoadsorption for accurate measurement, aiding research into immune complex roles.

    Area of Science:

    • Immunology
    • Biochemistry
    • Clinical Diagnostics

    Background:

    • Polymeric IgA-immune complexes play a role in various diseases.
    • Accurate measurement of these complexes is crucial for understanding their physiopathological significance.
    • Existing assays may lack specificity or simplicity.

    Purpose of the Study:

    • To develop a simplified, rapid, and reproducible assay for measuring polymeric IgA-immune complexes in biological fluids.
    • To validate the assay's specificity and reliability.

    Main Methods:

    • Utilized a standard Raji cell assay to determine multimeric IgA immune complexes.
    • Employed labeled secretory component for specific binding to polymeric IgA-immune complexes.
    • Incorporated prior IgM immunoadsorption to prevent false positives from IgM immune complexes.

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    Main Results:

    • The assay specifically binds labeled secretory component to polymeric IgA-immune complexes fixed to Raji cells.
    • Polymeric IgA immune complexes bind to Raji cells via complement breakdown products, not Fc receptors.
    • Successfully detected specific polymeric IgA-immune complexes in patients with IgA nephropathy and alcoholic liver disease, and in healthy subjects.

    Conclusions:

    • The developed assay is simple, rapid, and reproducible for detecting polymeric IgA-immune complexes.
    • This assay can aid in studying the physiopathological role of polymeric IgA-immune complexes in humans and animals.
    • The findings support the clinical relevance of measuring polymeric IgA-immune complexes.