Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Serological visualization of interleukin 2.

G Steinmann, P Conlon, S Hefeneider

    Science (New York, N.Y.)
    |June 10, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genetic polymorphism in <i>Methylenetetrahydrofolate Reductase chloride transport protein 6</i> (<i>MTHFR CLCN6</i>) gene is associated with keratinocyte skin cancer in a cohort of renal transplant recipients.

    Skin health and disease·2022
    Same author

    Influence of Various Factors on Circulating 25(OH) Vitamin D Concentrations in Dogs with Cancer and Healthy Dogs.

    Journal of veterinary internal medicine·2017
    Same author

    High-risk cutaneous malignancies and immunosuppression: Challenges for the reconstructive surgeon in the renal transplant population.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2017
    Same author

    The influence of inhaled anesthetic and body temperature on serum fentanyl concentrations with transdermally administered fentanyl in anesthetized dogs.

    Veterinary anaesthesia and analgesia·2017
    Same author

    Clinical manifestations of prune belly syndrome.

    Clinical medicine (London, England)·2016
    Same author

    Improving outcomes in patients with Acute Kidney Injury: the impact of hospital based automated AKI alerts.

    Postgraduate medical journal·2015
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    Morphological identification of Interleukin 2 (IL-2) was achieved using immunoperoxidase staining. This method distinguished IL-2 producer T cells from responder cells, revealing distinct cellular staining patterns for each cell type.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Interleukin 2 (IL-2) is a critical lymphokine functioning as a T-cell growth factor, essential for cellular immune responses.
    • Morphological identification of IL-2 has been a challenge in understanding immune cell interactions.

    Purpose of the Study:

    • To morphologically identify Interleukin 2 (IL-2) in immune cells.
    • To differentiate between IL-2 producer and responder T cells using immunocytochemical methods.

    Main Methods:

    • Immunoperoxidase staining was employed using a monoclonal antibody specific to IL-2.
    • Cytocentrifuge preparations of peripheral blood leukocytes stimulated with a T-cell mitogen were analyzed.
    • Cloned IL-2 producer and responder cell lines were utilized for detailed phenotyping.

    Related Experiment Videos

    Main Results:

    • IL-2 was successfully identified morphologically in stimulated leukocytes.
    • Distinct staining patterns differentiated IL-2 producer T cells (intense, ringlike cytoplasmic staining) from responder cells (less intensive, spotlike membrane staining).
    • Responder cells showed potential IL-2 capping phenomena on their membranes.

    Conclusions:

    • Immunoperoxidase staining provides a reliable method for the morphological identification and localization of IL-2 within immune cells.
    • Distinct cellular phenotypes of IL-2 producer and responder cells were elucidated, advancing the understanding of T-cell activation and communication.