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Nucleotide sequence of the Rasheed rat sarcoma virus oncogene: new mutations
Science (New York, N.Y.)
|July 8, 1983
Summary
Researchers sequenced the rat sarcoma virus oncogene (Ra-v-ras), revealing a distinct 29,000-dalton transforming protein (p29). This protein differs from Harvey murine sarcoma virus p21, with unique amino and carboxyl terminus mutations specific to rat sarcoma virus.
Area of Science:
- Molecular Biology
- Virology
- Oncology
Background:
- The Rasheed strain of rat sarcoma virus contains an oncogene responsible for cellular transformation.
- Understanding oncogene sequences is crucial for identifying viral oncogenes and their protein products.
Purpose of the Study:
- To determine the nucleotide sequence of the rat sarcoma virus oncogene (Ra-v-ras).
- To characterize the transforming protein encoded by Ra-v-ras and compare it to related viral proteins.
Main Methods:
- Nucleotide sequencing of the Ra-v-ras oncogene.
- Analysis of the encoded protein sequence and comparison with Harvey murine sarcoma virus p21.
Main Results:
- The Ra-v-ras oncogene encodes a 29,000-dalton transforming protein (p29).
- The p29 protein exhibits distinct amino-terminal sequences and additional carboxyl-terminal mutations compared to Harvey murine sarcoma virus p21.
- These sequence variations appear to be unique to the rat sarcoma virus.
Conclusions:
- The nucleotide sequence analysis reveals a novel transforming protein (p29) in the Rasheed rat sarcoma virus.
- The identified sequence differences suggest potential functional or regulatory distinctions between rat sarcoma virus and Harvey murine sarcoma virus.
- Further investigation is needed to elucidate the functional significance of these observed mutations in p29.