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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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Updated: Mar 25, 2026

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
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Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

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Enzyme modulator mediated immunoassay (EMMIA).

T T Ngo

    The International Journal of Biochemistry
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Enzyme modulator mediated immunoassay (EMMIA) offers a separation-free method for detecting analytes. This enzyme amplified immunoassay modifies enzyme activity, enabling sensitive detection without sample separation.

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    Area of Science:

    • Biochemistry
    • Immunochemistry
    • Assay Development

    Background:

    • Enzyme amplified immunoassays are crucial for sensitive analyte detection.
    • Homogeneous assays eliminate the need for separation steps, simplifying procedures.
    • Enzyme modulator mediated immunoassay (EMMIA) is a novel homogeneous immunoassay format.

    Purpose of the Study:

    • To detail the principle and development stages of EMMIA.
    • To discuss criteria for selecting suitable enzymes and modulators for EMMIA.
    • To present examples of EMMIA applications for diverse analytes.

    Main Methods:

    • Elaboration of the EMMIA principle involving analyte-labeled enzyme modulators and indicator enzymes.
    • Description of antibody-mediated abrogation of enzyme modulation.
    • Discussion of enzyme and modulator selection criteria.

    Main Results:

    • Detailed explanation of the EMMIA mechanism.
    • Criteria for enzyme and modulator selection provided.
    • Demonstration of EMMIA applicability across various analyte classes.

    Conclusions:

    • EMMIA is a viable separation-free, enzyme-amplified immunoassay.
    • The assay principle relies on analyte-modulated enzyme activity and antibody intervention.
    • EMMIA offers a versatile platform for sensitive analyte detection.