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Gluconeogenesis in chick embryo isolated hepatocytes.

A J Dickson

    The International Journal of Biochemistry
    |January 1, 1983
    PubMed
    Summary

    Chick embryo hepatocytes efficiently convert lactate to glucose, similar to adult birds. Hormonal regulation of this gluconeogenesis differs, with glucagon stimulating glycogenolysis but not glucose production from lactate.

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    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Cell Biology

    Background:

    • Hepatocytes are key in glucose homeostasis.
    • Understanding gluconeogenesis in embryonic development is crucial.
    • Chick embryo models offer insights into metabolic pathways.

    Purpose of the Study:

    • To investigate gluconeogenic precursor effectiveness in embryonic chick hepatocytes.
    • To assess hormonal regulation of gluconeogenesis in this model.
    • To compare embryonic and post-hatch gluconeogenic capacity.

    Main Methods:

    • Isolation of hepatocytes from 18-day-old chick embryos.
    • Measurement of gluconeogenic flux using [U-14C]lactate conversion to glucose.
    • Hormonal stimulation with glucagon and insulin.

    Main Results:

    • Lactate was the most effective gluconeogenic precursor, followed by pyruvate, alanine, glutamine, and glycerol.
    • Gluconeogenic precursor effectiveness was similar to post-hatch hepatocytes.
    • Glucagon stimulated endogenous glycogenolysis but not lactate gluconeogenesis.
    • Insulin did not affect gluconeogenesis.

    Conclusions:

    • Embryonic chick hepatocytes exhibit robust gluconeogenic capacity, comparable to adult models.
    • Hormonal regulation of gluconeogenesis in chick embryos differs from adult patterns.
    • Lactate is a primary substrate for gluconeogenesis in developing chick liver.

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