Use of cycloheximide for cultivating Mycobacterium lepraemurium in cell culture

A serial increase in the number of Mycobacterium lepraemurium with successful subcultures has been obtained in cell culture with cycloheximide treatment. The infected cells seldom floated off the culture vessel. They could be maintained and would support the bacillary multiplication in good condition for ten weeks or more without changing the medium frequently. An overall generation time of the intracellular bacilli up to the tertiary culture for the total period of 35 weeks was 22.1 days.