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Related Experiment Videos

Complement-activating immune deposits in systemic lupus erythematosus skin.

W R Gammon, C C Merritt, D C Henke

    The Journal of Investigative Dermatology
    |July 1, 1983
    PubMed
    Summary

    Immune deposits in lupus skin can activate complement, but not all deposits are equally potent. Functional assays reveal complement activation at the basement membrane zone, suggesting a role in disease.

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    Area of Science:

    • Immunology
    • Dermatology
    • Pathology

    Background:

    • Immune deposits at the cutaneous basement membrane zone are characteristic of systemic lupus erythematosus (SLE).
    • Previous studies suggested these deposits contain complement-activating immune complexes, but this was not functionally confirmed.
    • The ability of immune deposits to activate complement at the basement membrane zone in situ remained undetermined.

    Purpose of the Study:

    • To functionally assess complement activation by immune deposits in lupus skin biopsies.
    • To compare the sensitivity of functional assays versus direct immunofluorescence for detecting complement activation.
    • To investigate whether immune deposits can activate complement at the basement membrane zone in vitro.

    Main Methods:

    • Direct immunofluorescence for C3 to detect in vivo complement deposition.

    Related Experiment Videos

  • Leukocyte attachment assay to detect complement activation.
  • Indirect C3 binding immunofluorescence to measure complement activation in vitro.

    • Main Results:

    • Complement activation was detected at the basement membrane zone in some lupus skin biopsies with immunoglobulin deposits.
    • The capacity for complement activation varied quantitatively among different deposits.
    • Direct C3 immunofluorescence was less sensitive than functional assays for detecting complement-activating complexes.

    Conclusions:

    • Immune deposits in some lupus skin biopsies are functionally complement-activating complexes.
    • These complexes have the potential to activate complement at the basement membrane zone in vivo.
    • Functional assays may be valuable adjuncts to immunofluorescence for studying immune deposits in SLE.