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Localization of eosinophil granule major basic protein in chronic urticaria
The Journal of Investigative Dermatology
|July 1, 1983
Summary
Eosinophil degranulation, releasing cytotoxic major basic protein (MBP), was observed in chronic urticaria lesions. This suggests MBP may contribute to the development of chronic urticaria skin conditions.
Area of Science:
- Immunology
- Dermatology
- Pathology
Background:
- The specific role of eosinophils in skin disease pathogenesis remains unclear.
- Eosinophil granule major basic protein (MBP) is a potent cytotoxic molecule.
Purpose of the Study:
- To investigate eosinophil degranulation in chronic urticaria lesions.
- To determine if the cytotoxic major basic protein (MBP) is present outside eosinophils in these lesions.
Main Methods:
- Indirect immunofluorescence assay was performed on chronic urticaria tissue biopsies.
- Antibodies specific to MBP were used to detect its deposition.
- Formalin-fixed, paraffin-embedded tissue sections were analyzed.
Main Results:
- Evidence of eosinophil degranulation, indicated by MBP deposition, was found in 12 out of 28 biopsies.
- MBP deposition occurred on small blood vessel walls, dispersed granular material, and connective tissue fibers.
- Positive staining patterns varied among the patients.
Conclusions:
- These findings suggest a potential role for the cytotoxic eosinophil granule protein, MBP, in the pathogenesis of chronic urticaria.
- Eosinophil degranulation and MBP release may contribute to the evolution of chronic urticaria lesions.
