Thin-layer chromatography of some cationic dyes commonly used in histology

A number of cationic dyes commonly used in histology may be conveniently and effectively analysed using the thin-layer chromatographic system of Marshall and Lewis. Commercial samples of Acridine Orange, Crystal Violet, Janus Green B,Methyl Violet, Neutral Red, Pyronin B, Pyronin Y(G), Safranin, Victoria Blue B and Victoria Blue 4R have been analysed with this system. All have been found to be complex mixtures of coloured components. Chromatographic data on these components are presented.