Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kinetic interpretation of hepatic multiple-indicator dilution studies.

C A Goresky

    The American Journal of Physiology
    |July 1, 1983
    PubMed
    Summary

    This study uses a novel indicator dilution method to map liver processes. It reveals how different substances move through liver spaces and cells, distinguishing flow-limited from barrier-limited exchange.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Uptake of lactate by the liver: effect of red blood cell carriage.

    American journal of physiology. Gastrointestinal and liver physiology·2000
    Same author

    Kinetics of endothelin-1 binding in the dog liver microcirculation in vivo.

    The American journal of physiology·1999
    Same author

    Hepatic uptake of bromosulfophthalein-glutathione in perfused Eisai hyperbilirubinemic mutant rat liver: a multiple-indicator dilution study.

    The Journal of pharmacology and experimental therapeutics·1998
    Same author

    Costs and effectiveness of extracorporeal gallbladder stone shock wave lithotripsy versus laparoscopic cholecystectomy. A randomized clinical trial. McGill Gallstone Treatment Group.

    International journal of technology assessment in health care·1998
    Same author

    Carrier-mediated entry of 4-methylumbelliferyl sulfate: characterization by the multiple-indicator dilution technique in perfused rat liver.

    Hepatology (Baltimore, Md.)·1998
    Same author

    Endothelin-1 myocardial clearance, production, and effect on capillary permeability in vivo.

    The American journal of physiology·1997

    Area of Science:

    • Hepatology
    • Microcirculation Physiology
    • Pharmacokinetics

    Background:

    • Understanding liver function requires detailed knowledge of microcirculatory dynamics.
    • Quantifying exchange rates between blood, interstitium, and liver cells is crucial for drug delivery and disease assessment.

    Purpose of the Study:

    • To develop and apply a method for determining liver composition and process rates.
    • To differentiate between flow-limited and barrier-limited exchange mechanisms in the liver.

    Main Methods:

    • Single-injection, multiple-indicator dilution technique.
    • Analysis using a mathematical model of microcirculatory events.
    • Comparison with a vascular reference (labeled red blood cells).

    Main Results:

    • Interstitial materials enter Disse space in a delayed, flow-limited manner, with size exclusion.
    • Labeled water shows flow-limited distribution into liver cells.
    • Many substances exhibit barrier-limited exchange, with distinct throughput and returning tracer profiles.
    • Metabolic sequestration or biliary secretion affects tracer recovery and concentration gradients.

    Conclusions:

    • The indicator dilution method effectively characterizes liver microcirculation and cellular exchange.
    • Distinguishes flow-limited vs. barrier-limited transport, providing insights into substance distribution.
    • Reveals mechanisms influencing tracer dynamics, including sequestration and cell membrane transport.

    Related Experiment Videos