Characteristics of immobilized histamine for pyrogen adsorption

The characteristics of immobilized histamine for pyrogen adsorption were investigated. The adsorbent showed a high affinity for pyrogen at low ionic strength, at around neutral pH, at high temperature and at low flow-rates of a solution containing pyrogen. The adsorption capacity per millilitre of the adsorbent was 0.9 mg pyrogen. Immobilized histamine could be completely regenerated by washing with 0.2 M sodium hydroxide solution containing 10--30% ethanol followed by 1.5 M sodium chloride solution, or 0.2 M sodium hydroxide solution followed by 0.5% sodium deoxycholate solution, 0.2 M sodium hydroxide solution and 1.5 M sodium chloride solution.