Universal rosetting reagent for the detection of human cell surface markers

Area of Science:

• Immunology
• Cell Biology

Background:

• Monoclonal antibodies are widely used for detecting human cell surface markers.
• A universal, simple, and reproducible reagent is needed for these applications.

Purpose of the Study:

• To develop a versatile rosetting reagent for detecting human cell surface markers using mouse monoclonal antibodies.
• To evaluate the sensitivity and reliability of the developed reagent.

Main Methods:

• A rosetting reagent was developed using ox red blood cells, rabbit anti-ox RBC antibody, protein A, and rabbit anti-mouse Ig antibody.
• The reagent's binding specificity to mouse monoclonal antibody-coated human lymphocytes was assessed.
• Comparisons were made with immunofluorescence techniques using fresh and frozen peripheral blood lymphocytes.

Main Results:

• The developed rosetting reagent specifically binds to human lymphocytes coated with mouse monoclonal antibodies.
• The reagent demonstrated sensitivity comparable to immunofluorescence.
• Consistent results were obtained with both fresh and frozen peripheral blood lymphocytes.

Conclusions:

• The rosetting reagent is a practical, sensitive, and reliable tool for detecting human cell surface markers.
• Its versatility makes it particularly useful for large-scale studies, especially for human T cell markers.