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Human kidney preservation using machine perfusion followed by intracellular electrolyte flushing
The Journal of Urology
|August 1, 1983
Summary
Pulsatile machine perfusion (PMP) followed by cold storage can preserve kidney function for transplantation. This method, even with extended preservation, yielded functioning kidney grafts in human recipients.
Area of Science:
- Nephrology
- Transplantation Medicine
- Organ Preservation
Background:
- Transplant centers hesitate to accept kidneys preserved with pulsatile machine perfusion (PMP).
- Standard protocols for organ preservation vary, impacting organ acceptance and utilization.
Purpose of the Study:
- To evaluate the viability and function of human kidney grafts preserved using PMP followed by cold storage.
- To assess the feasibility of transplanting kidneys preserved with PMP despite initial reluctance from some centers.
Main Methods:
- Four human kidneys were preserved using PMP for 13-28 hours.
- Following PMP, kidneys underwent intracellular electrolyte flushing and cold storage for 13-36 hours.
- Kidneys were then transplanted, and recipient outcomes were monitored.
Main Results:
- All transplanted kidney grafts were functioning at 3 months post-transplantation.
- Recipients required temporary dialysis during the first post-transplant week.
- The average serum creatinine level at 1 month was 2.2 ± 1.1 mg/dL.
Conclusions:
- Pulsatile machine perfusion combined with cold storage is a viable organ preservation strategy.
- This combined approach can lead to successful kidney transplantation outcomes.
- Addressing transplant center reluctance is crucial for optimizing organ utilization.