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Influence of ionic strength on the antibody binding of MHC products
Ionic strength has a strong influence on the binding of human Ia and HLA(A,B,C) molecules by the corresponding antibodies. A control system, BSA-anti-BSA, was influenced only minimally. The effect on the binding of anti-beta 2-microglobulin was very small when isolated chains were used, but more pronounced when using whole HLA(A,B,C) molecules. An inverse relationship exists between degree of inhibition and antibody concentration.
Ionic strength has a strong influence on the binding of human Ia and HLA(A,B,C) molecules by the corresponding antibodies. A control system, BSA-anti-BSA, was influenced only minimally. The effect on the binding of anti-beta 2-microglobulin was very small when isolated chains were used, but more pronounced when using whole HLA(A,B,C) molecules. An inverse relationship exists between degree of inhibition and antibody concentration.