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[In vitro study of combinations between third-generation cephalosporins and aminoglycosides on Enterobacteriaceae]
Abstract:
Four cephalosporins (cefotaxime, moxalactam, cefoperazone and ceftriaxone) were tested in combination with four aminoglycosides (gentamicin, tobramycin, netilmicin and amikacin). We examined 16 clinically isolates of Enterobacteriaceae of which 8 were resistant to newer cephalosporins. The synergy studies was evaluated with a microtiter checkerboard broth dilution. Studies of synergy revealed that cephalosporins in combination with amikacin were often synergistic against third generation cephalosporins resistant strains. These results suggest that amikacin may be facilitate the cephalosporin antibacterial activity.
Insights
Combining cephalosporins with amikacin showed synergistic effects against resistant Enterobacteriaceae. This suggests amikacin enhances cephalosporin activity, offering a potential strategy against challenging bacterial infections.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Emerging resistance to advanced cephalosporins in Enterobacteriaceae poses a significant clinical challenge.
- Understanding synergistic antibiotic combinations is crucial for overcoming antimicrobial resistance.
Purpose of the Study:
- To evaluate the synergistic potential of combining four cephalosporins with four aminoglycosides against clinical isolates of Enterobacteriaceae.
- To identify effective antibiotic combinations for combating cephalosporin-resistant bacterial strains.
Main Methods:
- Synergy testing was performed using a microtiter checkerboard broth dilution method.
- Sixteen clinical isolates of Enterobacteriaceae, including eight resistant to newer cephalosporins, were analyzed.
Main Results:
- Cephalosporin and amikacin combinations demonstrated frequent synergy against strains resistant to third-generation cephalosporins.
- Specific synergistic interactions were observed, highlighting amikacin's role in potentiating cephalosporin efficacy.
Conclusions:
- Amikacin may facilitate and enhance the antibacterial activity of cephalosporins.
- The combination of cephalosporins with amikacin presents a promising therapeutic approach for treating infections caused by resistant Enterobacteriaceae.