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Beta-cell tropin: synthesis and biological activity.
Biochemical and Biophysical Research Communications
|July 29, 1983
Summary
Researchers synthesized beta-cell tropin, an insulin secretagogue from obese mice, identifying it as part of ACTH. The synthetic version matches natural beta-cell tropin in all tested properties.
Area of Science:
- Endocrinology
- Neuroscience
- Peptide Chemistry
Background:
- Beta-cell tropin, an insulin secretagogue, is released by the neuro-intermediate lobe of obese (ob/ob) mice.
- The structure of beta-cell tropin has been identified as the 22-39 moiety of adrenocorticotropic hormone (ACTH).
Purpose of the Study:
- To describe a method for the synthesis of beta-cell tropin.
- To confirm the identity and properties of synthetic beta-cell tropin.
Main Methods:
- Mild solid-phase peptide synthesis.
- Preparative high-performance liquid chromatography (HPLC).
- Fast Atom Bombardment mass spectrometry (FAB-MS).
Main Results:
- A method for preparing the octadecapeptide beta-cell tropin was successfully developed.
- The molecular weight of the synthetic peptide was confirmed using FAB-MS.
- Synthetic beta-cell tropin demonstrated indistinguishable chromatographic, antigenic, and biological properties compared to the natural form.
Conclusions:
- The synthesis of beta-cell tropin is feasible using described solid-phase procedures.
- Synthetic beta-cell tropin can serve as a reliable substitute for natural beta-cell tropin in research and potentially therapeutic applications.
- This work validates the structural determination of beta-cell tropin as the 22-39 moiety of ACTH.