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Updated: Aug 12, 2026
10:57
Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
Published on: February 3, 2017
A technique for preserving aerial fungal structures for scanning electron microscopy
Canadian Journal of Microbiology
|June 1, 1983
Summary
This study introduces a new method using vapor fixation and diffusion dehydration to prepare delicate fungal samples for scanning electron microscopy, preserving their structures effectively.
Area of Science:
- Mycology
- Microscopy techniques
Background:
- Scanning electron microscopy (SEM) requires sample dehydration, which can damage delicate biological structures.
- Fungal structures are particularly susceptible to distortion during traditional preparation methods.
Purpose of the Study:
- To develop a gentle preparation technique for SEM of fungi.
- To preserve the intricate morphology of fungal specimens.
Main Methods:
- Utilized vapor fixation for initial sample stabilization.
- Employed a simple vapor diffusion apparatus for dehydration.
- Applied the technique to fungi cultured on agar and natural substrates.
Main Results:
- Minimized disturbance of delicate fungal structures.
- Successfully preserved fungal morphology during SEM preparation.
- Demonstrated applicability across different growth media.
Conclusions:
- The described technique offers a reliable method for SEM preparation of fungi.
- This approach enhances the quality of ultrastructural imaging in mycology.
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