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Mutagenicity testing of steroids obtained from bile acids and cholesterol
Abstract:
Twenty bile acid or cholesterol derivatives were tested for mutagenicity by the Ames spot test procedure against five Salmonella typhimurium strains in the presence and absence of rat liver microsomes. No strong mutagens were identified, but four of the compounds increased the number of revertant colonies observed. Three of these compounds were selected for dose response experiments using the Ames plate incorporation assay procedure with strain TA 1538. No positive results were obtained. These results suggest that none of the twenty bile acid derivatives tested is likely to be the initiating agent in the aetiology of colon cancer.
Insights
This study investigated bile acid derivatives for mutagenicity using the Ames test. No compounds were identified as mutagens, suggesting they do not initiate colon cancer.
Area of Science:
- Biochemistry
- Toxicology
- Carcinogenesis
Background:
- Bile acids and cholesterol derivatives are implicated in colon cancer etiology.
- Mutagenic potential of these compounds requires thorough investigation.
Purpose of the Study:
- To assess the mutagenicity of twenty bile acid or cholesterol derivatives.
- To determine if these compounds could be initiating agents in colon cancer.
Main Methods:
- Ames spot test against five Salmonella typhimurium strains.
- Testing in the presence and absence of rat liver microsomes.
- Dose-response experiments using Ames plate incorporation assay with strain TA 1538 for selected compounds.
Main Results:
- No strong mutagens were identified among the twenty tested compounds.
- Four compounds showed a slight increase in revertant colonies.
- Dose-response experiments with three selected compounds yielded no positive mutagenicity results.
Conclusions:
- The tested bile acid derivatives are unlikely to be mutagenic.
- These compounds are unlikely to be the initiating agents in the etiology of colon cancer.