Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An internally referenced test strip immunoassay for morphine.

D J Litman, R H Lee, H J Jeong

    Clinical Chemistry
    |September 1, 1983
    PubMed
    Summary

    This study introduces a rapid, internally referenced immunochemical test-strip for morphine detection. The enzyme-channelling immunoassay provides reliable results by minimizing interference and standardizing the assay.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Link between Hyperinsulinemia and Periodontitis in Diabetics.

    Journal of dental research·2025
    Same author

    Association of prior treatment with nitrogen-containing bisphosphonates on outcomes of COVID-19 positive patients.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2023
    Same author

    Bisphosphonate holidays: using cost-effectiveness analysis for the "yes, but" questions.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2021
    Same author

    At-home determination of 24-h urine sodium excretion: Validation of chloride test strips and multiple spot samples.

    Autonomic neuroscience : basic & clinical·2021
    Same author

    Therapeutic effects of Artemisia scoparia Waldst. et Kitaib in a murine model of atopic dermatitis.

    Clinical and experimental dermatology·2018
    Same author

    Clinical Significance of Macrophage Polarization in Antibody-Mediated Rejection of Renal Allograft.

    Transplantation proceedings·2018

    Area of Science:

    • Biochemistry
    • Immunochemistry
    • Analytical Chemistry

    Background:

    • Rapid drug detection is crucial for clinical and forensic applications.
    • Existing immunoassays may suffer from variability due to environmental factors or sample interference.
    • Development of internally standardized assays can improve reliability.

    Purpose of the Study:

    • To develop and validate an internally referenced immunochemical test-strip for the rapid detection of morphine.
    • To utilize an enzyme-channelling immunoassay for enhanced sensitivity and specificity.
    • To establish a reliable and standardized method for morphine screening.

    Main Methods:

    • An enzyme-channelling immunoassay technique using a glucose oxidase-horseradish peroxidase enzyme pair.

    Related Experiment Videos

  • Test strips with two active surfaces: an indicator pad (drug antibody) and a reference pad (anti-peroxidase).
  • A 10-minute assay protocol involving sample incubation, followed by developer solution incubation.

    • Main Results:

    • The assay generates an insoluble, colored reaction product on the test-strip surface.
    • Color development on the indicator pad is inhibited by the presence of morphine.
    • The reference pad normalizes for variations, allowing for accurate positive/negative scoring.

    Conclusions:

    • The internally referenced test-strip offers a rapid and reliable method for morphine detection.
    • The enzyme-channelling approach enhances assay performance and reduces variability.
    • This method provides a standardized platform for drug screening with improved accuracy.