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Summary
Evaluating factor VII activation is crucial for understanding thrombotic risk. New combined methods and studies on activated factor VII show its potential to activate factor X, even at low concentrations, suggesting thrombotic risks in circulation.
Area of Science:
- Biochemistry
- Hematology
- Thrombosis Research
Background:
- Direct quantification of activated factor VII (factor alpha-VIIa) is currently unavailable.
- Assessing factor VII activation requires indirect methods to evaluate its activity state in plasma.
Purpose of the Study:
- To describe techniques for evaluating factor VII activity state in plasma.
- To investigate the potential thrombotic properties of activated factor VII.
Main Methods:
- Simultaneous assays: factor VII coagulant activity (VIIc), factor VII antigen (VIIag), coupled amidolytic factor VII assay (VIIam), and bovine tissue thromboplastin assay (VIIbt).
- Calculation of factor VII activation using ratios: VIIc/VIIag, VIIc/VIIam, or VIIbt/VIIc.
- Assessing the potency of activated factor VII to activate factor X in the presence of phospholipids, lipids, and calcium ions.
Main Results:
- Combined methods allow for the calculation of factor VII activation levels.
- Activated factor VII demonstrates significant potency in activating factor X.
- Even low concentrations of activated factor VII can activate factor X under specific conditions.
Conclusions:
- The described combined methods provide a means to assess factor VII activation.
- Activated factor VII possesses potential thrombotic properties.
- Factor VII activation may contribute to thrombosis even without exposed tissue thromboplastin in circulation.