A rinsing and incubation chamber used for immunocytochemistry of vibratome sections

This paper describes the construction and use of a washing/incubation chamber that may be used for peroxidase-antiperoxidase immunocytochemistry of vibratome sections. The advantages of the chamber include: all sections are processed simultaneously with exception of exposure to the primary antiserum; the exposure of the sections to DAB substrate can be precisely controlled; and also the sections are not directly manipulated during the procedure resulting in a much greater integrity of the tissue. Use of the chamber reduces the amount of labor normally required to wash the sections throughout the procedure. Application of this system to the development and standardization of quantitative immunocytochemical techniques is also discussed.